Journal: Journal of Pain Research
Article Title: Electroacupuncture Alleviates KOA-Induced Pain and Cartilage Degeneration via NGF/TrkA Pathway
doi: 10.2147/JPR.S560506
Figure Lengend Snippet: Specific blockade of NGF/TrkA signaling ameliorates pain and cartilage degeneration in KOA model rats. ( A ) Schematic illustration of model establishment, MNAC13 administration, behavioral testing, and tissue collection. Arrows in the schematic indicate the time points for model induction. ( B and C ) Changes in paw withdrawal threshold (PWT) in the saline, MIA, MIA + MNAC13, and MIA + EA groups (per group, n = 7). *p < 0.05, **p < 0.01, ***p < 0.001 vs saline group; # p < 0.05, ### p < 0.001 vs MIA group. ( D and E ) Changes in weight-bearing asymmetry (WBA) in the saline, MIA, MIA + MNAC13, and MIA + EA groups (per group, n = 7). **p < 0.01, ***p < 0.001 vs saline group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs MIA group. ( F ) Representative toluidine blue-stained images of cartilage from the saline, MIA, MIA + MNAC13, and MIA + EA groups. Scale bar = 500 µm. ( G ) Quantification of cartilage degeneration scores in the four groups (per group, n = 3). ***p < 0.001.
Article Snippet: Goat anti-β-NGF antibody (3 μg/mL, R&D Systems, USA), goat anti-TrkA antibody (3 μg/mL, R&D Systems, USA), rabbit anti-PGP9.5 antibody (1:800, Abcam, UK), rabbit anti-TNF-α antibody (1:1000, Abcam, UK), rabbit anti-IL-1β antibody (1:1000, Abcam, UK), rabbit anti-CGRP antibody (1:400, Cell Signaling Technology, USA), mice anti-NF200 antibody (1:200, Cell Signaling Technology, USA), Isolectin B4 (BSI-B4, 1:1000, Sigma-Aldrich, USA).
Techniques: Saline, Staining